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CFSE Dye Dilution Proliferation Assay The CFSE (carboxyfluorescein diacetate succinimidyl ester) dye dilution proliferation assay is based on the covalent labeling of intracellular proteins by a cell-permeant fluorescent dye that becomes fluorescent upon intracellular ester cleavage and then is stably retained within cells
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A claim of the form "this fragment retains function X but not function Y" contains two assertions, and both require demonstration
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