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In addition, 50 μl of 60 μM XN (T3342, Taojiao, China), 10 mM 3-Methyladenine (HY-19312, MCE, China), and PBS (BL601A, Biosharp, China) were injected into the right dorsal side of C57 mice after plucking at 8 weeks of age [21]

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Key Research Areas for Glutathione Intracellular redox status measurement — GSH/GSSG ratio as a primary cellular oxidative stress index Oxidative stress induction and rescue assays — GSH depletion (buthionine sulfoximine, BSO) and repletion studies Glutathione peroxidase (GPx) and glutathione reductase (GR) enzyme activity assays Glutathione S-transferase (GST) detoxification pathway research — xenobiotic and electrophile conjugation studies S-glutathionylation and redox-regulated post-translational modification studies Nrf2/ARE pathway research — transcriptional regulation of GSH synthesis and antioxidant gene expression Mitochondrial GSH pool studies — mtGSH depletion, mitochondrial oxidative damage, and mtDNA protection research Cancer cell biology — GSH-dependent drug resistance, ferroptosis pathway research, GSH-targeted therapeutic strategy studies Neurodegeneration pathway research — GSH depletion in dopaminergic and other neuronal cell models Immune cell function — GSH regulation of T-cell proliferation, cytokine production, and inflammatory signalling Lipid peroxidation and ferroptosis pathway studies — GPx4-GSH axis research GCL/GS enzyme activity and GSH biosynthesis regulation studies in vitro What Do Studies Say About Glutathione