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Furthermore, achieving this outcome is feasible by administering vitamin C supplements (99)

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After 3–4 days in culture, slices were transferred to an artificial cerebrospinal fluid (ACSF) containing (in mM): 130 NaCl, 2.5 KCl, 2.2 CaCl 2 , 1.5 MgCl 2 , 10 D-glucose, 10 Hepes (pH 7.35, osmolarity adjusted to 300 mOsm)