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Dark circles under your eyes—sound familiar? 😴 From lack of sleep to genetics, there are several culprits behind those pesky shadows. In our one-minute DocShorts video, our Consultant Dermatologist Dr Shweta Manchanda

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To reduce background staining, the cells were quenched for 10min with 50nM glycine in TBS and blocked for 30min with 5% Normal Horse Serum (NHS) or Normal Goat Serum (NGS) in 0,2% gelatin-TBS solution, depending on the isotype of the secondary antibody