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S.DerooB.et al (1998)
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Learn more: Reverse transcriptase properties Primer design and controls for accurate quantitation in RT-qPCR Primer design PCR primers for the qPCR step of RT-qPCR should ideally be designed to span an exon-exon junction, with one of the amplification primers potentially spanning the actual exon-intron boundary ( Figure 4 )
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