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One study showed that pUL14 translocates to the nucleus and nucleolus upon stress, such as heat shock and osmotic shock (Yamauchi et al., 2002a, 2002b, 2003)
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A single grown colony was inoculated into 5 ml of the YPD liquid medium in a 25 ml glass test tube closed with a tapered plug closure made of silicone sponge
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