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The competition between NA methylation and NAD + salvage indicates that NNMT might limit fuel oxidation and promote fat storage, because if the activity or expression of NNMT is high, then, NA might not be salvageable, thus limiting the NAD + -dependent processes [16]

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Regional legislation and guidance (e.g., European Directive 2010/63/EU) may stipulate the minimal conditions that need to be met when conducting experiments in animals, but there is a wealth of information from other recognised experts on topics such as euthanasia and recognition of pain that may be useful to ensure the appropriate implementation of humane endpoints (Hawkins et al