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Immunocytochemistry Unless otherwise stated, cells previously plated in 96 wells plates for imaging were rinsed in PBS, fixed for 15 min in 4% PFA/PBS, rinsed 3 times in PBS, permeabilized for 5 min with 0.15% Triton X-100/PBS (or 0.05% NP-40 where indicated), rinsed again 3 times in PBS, and incubated for 30 min in blocking solution (5% normal goat serum in PBS)

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