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The nucleus was stained with 0.5 μg/mL DAPI
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Membranes were incubated overnight at 4°C with the following antibodies: Phospho-Rb (Ser807/811) (D20B12) XP Rabbit mAb (Cell Signaling Technology #8516), Purified Mouse Anti-Human Retinoblastoma Protein Clone G3–245 (BD Biosciences 554136), Monoclonal ANTI-FLAG M2 antibody produced in mouse (Sigma-Aldrich F1804), E2F-1 Antibody (Cell Signaling Technology #3742), and mouse monoclonal β-Actin Antibody N-21 (Santa Cruz Biotechnology sc-130656)
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